Coding
Part:BBa_K2142004
Designed by: Lukas Rieder Group: iGEM16_NAWI-Graz (2016-10-07)
Dam methylase
This coding sequence was used to generate a higher mutation rate. Norma this protein is used as repairing system. With overexpression its possible to reach a mutation rate which is 50x more than normal. Mutation will occur on plasmid and genom.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 306
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |