Composite
ExpSubE

Part:BBa_K2020002:Design

Designed by: Nicola Freyer, Lea Steinbeck, Svenja Meyer, Alexander Deitert   Group: iGEM16_Aachen   (2016-10-05)
Revision as of 16:38, 5 October 2016 by Registry (Talk | contribs)

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expression system for subtilisin E in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT.


Source

The sequences of the BioBrick parts were obtained through the Registry. The construct was ordered at IDT. add links

References