Coding

Part:BBa_K2009357:Design

Designed by: Kaiyue Ma   Group: iGEM16_USTC   (2016-09-14)
Revision as of 13:48, 14 September 2016 by Registry (Talk | contribs)

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SUP35


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 522


Design Notes

The SUP35NM gene we used is provided by Dong Men, PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences, so that the sequence is not quite the same as the existing sequence in the Parts Registry, for a lot of mutations have been done. The standardization of this gene is did by ourselves by adding the Biobrick prefix and suffix to its ends and doing a site mutation to eliminate the PstI cutting site inside it.


Source

PCR from the plasmid provided from Dong Men , PhD of Wuhan Insititue of Virology of Chinese Academy of Sciences

References