Composite

Part:BBa_K2036029:Design

Designed by: Zhi Zeng   Group: iGEM16_HUST-China   (2016-09-24)
Revision as of 09:32, 3 October 2016 by Shareycheng (Talk | contribs) (→‎Design Notes)


Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1765
    Illegal BsaI.rc site found at 2436


Design Notes

ABF2 can serve as transcriptional activator to initiate GFP expression as a generator.

Source

Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-tag

References