Composite

Part:BBa_K2036030

Designed by: Zhi Zeng   Group: iGEM16_HUST-China   (2016-09-24)
Revision as of 09:21, 3 October 2016 by Shareycheng (Talk | contribs)


PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag

ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit.

Fig1:bi-stable function of Signal Filter Eukaryotic circuit

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BglII site found at 3824
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal BamHI site found at 1816
    Illegal BamHI site found at 2473
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3756
    Illegal AgeI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5268
    Illegal BsaI.rc site found at 5939


[edit]
Categories
Parameters
None