Part:BBa_K2092009
PalcA(var) + RBS (B0034) +amilCP
PalcA is one of the strongest inducible promoters in Aspergillus nidulans commonly used to overexpress proteins [1]. It has been shown that PalcA promoter is also functional in monocotyledonous plant sugar cane [2] and Escherichia coli (E.coli) [3]. Its transcriptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine.
The native PalcA consists of 3 AlcR binding sites. The number and position of the AlcR binding sites on the PalcA are crucial in determining its transcriptional activation strength. It has also been shown that each AlcR target in the PalcA contributes differently to the activation of the downstream protein expression [1]. In contrast to the native construct (three AlcR binding sites abc), this variant consists only two binding sites bc. Previous study on sugarcane has shown that there is a 70% decrease in promoter strength for PalcA with only binding sites bc [2].
This part allows detection of ethanol by coupling it with another composite part, <a href="https://parts.igem.org/Part:BBa_K2092008"> BBa_K2092008</a>, which constitutively expresses its transcription factor, AlcR. Blue color development can be seen in presence of ethanol.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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