Coding

Part:BBa_K2010000

Designed by: William Cho   Group: iGEM16_Harvard_BioDesign   (2016-06-17)
Revision as of 19:17, 2 October 2016 by Williamcho (Talk | contribs)


PETase (PET-degrading enzyme, origin I. sakaiensis)

PETase is the PET(poly(ethylene terephtalate))-degrading enzyme first identified in Ideonella sakaiensis. This sequence is the E. coli K12 optimized DNA sequence for PETase.

This part includes a promoter, RBS, and PETase fused with a His-tag, for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 426


[edit]
Categories
//function/degradation
Parameters
protein