RNA

Part:BBa_K2020042:Design

Designed by: Andrea Hoeltken, Vroni Czotscher, Carolina Bonerath   Group: iGEM16_Aachen   (2016-10-02)
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Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.

Team Aachen 2016 ( http://2016.igem.org/Team:Aachen ) used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter.


Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA

References