Coding
D-LDH*

Part:BBa_K2042008:Design

Designed by: Amir Pandi   Group: iGEM16_Evry   (2016-08-30)
Revision as of 18:20, 1 October 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)


Engineered D-Lactate dehydrogenase (D-LDH*)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 630
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for Pseudomonas putida, three amino acid mutation were introduce (D176S, I177R, F178T)

Source

Synthetic synthesis

References