Regulatory

Part:BBa_K2092002

Designed by: Hui Wen Chong   Group: iGEM16_Manchester   (2016-10-01)
Revision as of 15:05, 1 October 2016 by Registry (Talk | contribs)

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PalcA, improved alcR inducible promoter from A. nidulans

The alcA promoter is one of the strongest inducible promoters in Aspergillus nidulans. Its transciptional activation is dependent on the binding of its positive transcriptional regulator AlcR with various substrates that employ a hydroxyl group, for example ethanol and threonine. The native AlcA promoter consists of 3 AlcR binding sites. The number and position of the AlcR binding sites on the alcA promoter are crucial in determining its transcriptional activation strength. It has also been shown that each AlcR target in the alcA promoter contributes differently to the activation of the downstream protein expression.

This part is an improved BioBrick previously characterised by 2011 iGEM DTU-Denmark. Sequencing results showed that two Prefix/Suffix restriction sites XbaI and SpeI were absent in the original part. Hence the two missing restriction sites were added using PCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None