Coding

Part:BBa_K401001:Experience

Designed by: Daniel Tamarit   Group: iGEM10_Valencia   (2010-10-21)
Revision as of 01:17, 1 October 2016 by Verweile (Talk | contribs)


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Please enter how you used this part and how it worked out.

Applications of BBa_K401001

User Reviews

UNIQ7f565561df9f36cb-partinfo-00000000-QINU UNIQ7f565561df9f36cb-partinfo-00000001-QINU

Improvement by USTC 2016


Our part is Part: BBa_K2009357 .We construted this composite by ligating GAL1 promoter + Kozak sequence (Part:BBa_J63006) and (2) GFP (Part:BBa_E0040). However, the ATG inside the KOZAK sequence won't be in the same reading frame as the ATG of the downstream coding sequence, so a frame-shift mutation is inevitable. To solve this problem, we add a base pair after the Kozak sequence so that the ATG can be in the same reading frame and the GFP can be expressed properly. What’s more, this part become “ready to use”, which means the GFP sequence can be directly altered by other functional parts and the sequence will be expressed properly.