Project

Part:BBa_K2036015

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)
Revision as of 05:13, 29 September 2016 by Shareycheng (Talk | contribs)


RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([ https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013 ]) and pRE-RBS-GFP-LVAssrAtag ( [ https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011 ]) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.

Fig1:CII and pRE interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1770


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Categories
Parameters
None