Coding

Part:BBa_K1895998

Designed by: Jake Burton   Group: iGEM16_Newcastle   (2016-07-21)
Revision as of 11:09, 21 July 2016 by Registry (Talk | contribs)

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SmtA metallothionein and LVA tag.

This is part BBa_K519010, SmtA which is a metallothionein that can bind to heavy metal ions. This variant has an LVA degradation tag before the stop codon which reduces its half-life in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 93
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 93
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 93
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None