Composite

Part:BBa_K1895999

Designed by: Jake Burton   Group: iGEM16_Newcastle   (2016-07-21)
Revision as of 18:28, 27 September 2016 by Jakeb (Talk | contribs) (Add description and basic biology of Arabinose controlled resistor.)


Arabinose controlled 'resistor'

As part of our iGEM project we have attempted to engineer E. coli to behave like a variable resistor. We hoped to do this by using E. coli to vary the amount of free ions in an electrolyte. Ion uptake will be controlled by the expression of a protein, smtA. SmtA is a metallothionein that can bind to heavy metal ions like cadmium (II), Zinc (II) and Copper (II).

In this construct we have placed smtA under the control of an AraC regulated promoter allowing the expression of smtA to be controlled by the addition or removal of arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 338
    Illegal AgeI site found at 674
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None