Composite

Part:BBa_K2036030:Design

Designed by: Zhi Zeng   Group: iGEM16_HUST-China   (2016-09-24)
Revision as of 09:52, 24 September 2016 by Registry (Talk | contribs)

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PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3756
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
    Illegal BglII site found at 3824
    Illegal BamHI site found at 661
    Illegal BamHI site found at 806
    Illegal BamHI site found at 918
    Illegal BamHI site found at 1816
    Illegal BamHI site found at 2473
    Illegal XhoI site found at 65
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3756
    Illegal AgeI site found at 48
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5268
    Illegal BsaI.rc site found at 5939


Design Notes

PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-tag


Source

PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-tag

References