Part:BBa_K1871002

Alpha amylase with mating factor alpha secretion

This is the alpha amylase from Bacillus amyloliquefaciens, flanked by Kozak sequence, mating factor alpha secretion sequence, and the ADH1 terminator. This part is controlled by the minimal cyc promoter.

Minimal cyc promoter

This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.

Kozak Sequence

This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

Mating Factor Alpha Secretion Sequence

This part is the secretion signal from yeast α-mating factor, and directs the secretion of the produced protein. This allows the exportation of the protein.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, alpha amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 105
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1497
  Illegal NgoMIV site found at 1512
  Illegal AgeI site found at 1815
  
• 1000
  COMPATIBLE WITH RFC[1000]