Coding

Part:BBa_K2042002

Designed by: Maria Bargués i Ribera   Group: iGEM16_Evry   (2016-09-02)
Revision as of 16:51, 4 September 2016 by RATOVOMANANA (Talk | contribs)


Pseudomonas sp. MBEL 6-19 PHA synthase 1

This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.) The sequence is codon optimized for Pseudomonas putida


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/pputida
Parameters
None