Coding
Pct

Part:BBa_K2042000:Design

Designed by: Toky RATOVOMANANA   Group: iGEM16_Evry   (2016-08-19)
Revision as of 19:31, 29 August 2016 by RATOVOMANANA (Talk | contribs) (→‎Design Notes)


Propionate CoA transferase for P. putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1081
    Illegal BsaI site found at 1552


Design Notes

The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to create Polylactic acid (PLA). Lactate can also act as acceptors for the enzyme. In our project, the enzyme will catalyze the reaction: acetyl-CoA + lactate <--> acetate + Lactyl-CoA.

Source

The wild type sequence is from Clostridium propionicum genome.


References