Coding

Part:BBa_K2030002:Design

Designed by: John Hellgren   Group: iGEM16_Chalmers_Gothenburg   (2016-08-17)
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Acetate kinase (ackA) from Synechocystis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The complete sequence of the genome of Synechocystis sp. PCC 6803(Genbank accession number NC_000911) was used to design primers for amplification of this gene.

FW primer: ATGAAATTCCTGATTCTCAATGC

RV primer: AGCCTAATTCAACATTTATCTTCAC

The reverse primer was designed to also include 50 bp downstream of the gene as a potential native terminator.


Source

Genomic DNA from Synechocystis sp. PCC 6803

References