Coding

Part:BBa_K2180009

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-05)
Revision as of 20:19, 5 August 2016 by Registry (Talk | contribs)

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LacI-LVA for B. subtilis

This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008 We delete the Fur Box in order to use it like a normal promoter. The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None