Coding
Part:BBa_K2010001:Design
Designed by: William Cho Group: iGEM16_Harvard_BioDesign (2016-06-17)
ompT + PETase (PET-degrading enzyme, origin I. sakaiensis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 522
Design Notes
This sequence was optimized for E. coli K12 using the Codon Optimization Tool from the IDT website.
Source
This part comes from Ideonella sakaiensis, identified in "A bacterium that degrades and assimilates poly(ethylene terephthalate)" (Yoshida et al. 2016).