Intermediate

Part:BBa_K1904003

Designed by: Enrique Chimal Juarez, Roger Milton Rubio Sánchez, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-07)

T4 Holin with RBS and terminator.

This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None