Protein_Domain

Part:BBa_M36142:Experience

Designed by: Tatyanna Dadabbo & Richard Hojel & Soraya Karimi   Group: Stanford BIOE44 - S11   (2015-10-22)
Revision as of 05:46, 5 December 2015 by Tdadabbo (Talk | contribs) (→‎User Reviews)

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Applications of BBa_M36142

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UNIQec2bb04c07dee08d-partinfo-00000000-QINU UNIQec2bb04c07dee08d-partinfo-00000001-QINU 12/04/15

This DNA sequence for the Mature EP-B2 was the correct sequence for the protein. When conducting a fluorescence assay for the protein in lysed cells, the results proved that the correct protein was being cleaved, therefore the mature EP-B2 protein was present. The assay was successful as the mature EP-B2 protein was necessary to cleave the fluorescent part of the quencher. When designing our constructs, we chose to make two constructs, one with the mature EP-B2 sequence and one with the mature EP-B2 sequence. We were testing whether the inhibitor sequence in the Pro EP-B2 was necessary for the protein to be made. Given our successful results for the Mature EP-B2 (with no inhibitor sequence) from the lysed cells fluorescence assay, we concluded that the inhibitor sequence is not necessary for the protein to be made.