Composite
Part:BBa_M36580:Design
Designed by: Elisa Vidales Group: Stanford BIOE44 - S11 (2015-10-22)
LasR/PAI complex sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Added preceding spacing to qsc102 promoter palindromic binding sequence, as well as spacing flanking the RBS in order to optimize binding.
Source
qsc102 promoter: Boxed region from Figure 1 of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ Whiteley spacing: Region following boxed region to bolded A of http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/ BBa_0030 strong RBS: partsregistry.org GFP coding sequence: BIOE44 pComet DNA sequence