Coding

Part:BBa_K1689016:Design

Designed by: Wei weijia   Group: iGEM15_Peking   (2015-09-16)
Revision as of 03:00, 17 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


dCas9-F[1,2]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
    Illegal BamHI site found at 3429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dCas9 fusion protein coding sequence is too long, so you'd better amplify it separately.


Source

E.coli

References