Coding
Part:BBa_K1689015:Design
Designed by: Wei weijia Group: iGEM15_Peking (2015-09-16)
F[1,2]-dCas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1441
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal BamHI site found at 3720 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There exists a protein linker: GGGGS*2, the GC% of it is high, so you'd better keep in mind when using Gibson Assembly; There is an EcoRI restriction site in dCas9, we have eliminated it by site mutation, you can use our part directly or you need pay attention to your dCas9 sequence.
Source
E.coli