Generator

Part:BBa_K1741013

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 08:06, 27 September 2015 by Michal.michalak (Talk | contribs)

sfGFP under proC promoter with a hairpin in 5'UTR

A hairpin introduced to 5’UTR slightly lowers transcription/translation. We are planning to extended it to use it as a thermometer, as for now we do not see temperature dependence.

UAMpoznanproChairpin.jpg

This is the potential hairpin structure.


Results

Legend:

proC [BBa_K1741012]

proC with a hairpin in 5'UTR [BBa_K1741013]

proD [BBa_K1741014]


Teamuamprocdiffsugarsgraph.png

Teamuampoznanprochairpindiffsuagrsgraph.png

Teamuampoznanproddiffsugarsgraph.png

The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.

We observed that proD is up to three times stronger than proC. We need to enhance the hairpin in proC with 5'UTR hairpin to stop the translation in 23° C.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI.rc site found at 232


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