![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1741004
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
sfGFP under melibiose promoter (improved 5'UTR)
Design
Legend:
MelS [BBa_K1741004]
MelWT [BBa_K1741003]
Results
Legend:
MelS [BBa_K1741004]
MelWT [BBa_K1741003]
The sfGFP fluorescence was measured using Tecan fluorometer.
We also checked the tightness of our promoters.
All of our promoters are induced only by their respective sugars with a small exception of xylose promoters being slightly induced by arabinose and arabinose promoters being slightly induced by xylose. All of our promoters are repressed by glucose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 207
[edit]
Categories
Parameters
None |