![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K1741003
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
sfGFP under melibiose promoter
MelR chromosomal copy of MelR is sufficient to activate the melibiose melAB promoter copied from E. coli K12 chromosome when melibiose is added to M9 minimal medium or to a rich medium 2xLB (0,4%). In both media induction is much weaker than of arabinose or rhamnose induced promoters.
Characteristics and Results
Legend:
MelS [Ba_K1741004]
MelWT [Ba_K1741003]
The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 154
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 206
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Categories
Parameters
None |