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Part:BBa_K1741008
sfGFP under promoter xylA1 with improved 5'UTR
=Design
Legend:
XylWT (XylA) [BBa_K1741007]
XylA1 [BBa_K1741008]
XylS [BBa_K1741009]
XylF [BBa_K1741010]
Genes involved in catabolism of xylose in E.coli are organized into two transcription units which are driven by two promoters - XylA and XylF. In order to increase the expression of a protein under our XylA1 promoter we have modified the 5'UTR of the XylWT promoter so it was similar to that of proD [BBa_K1741014], a strong constitutive promoter. In some experiments it seems to be slightly stronger than original XylA promoter from part BBa_K1741007.
The next step was to shorten the promoter by removing its XylF part while keeping the modified UTR. Finally we tested the XylF promoter upstream of sfGFP. XylS is the strongest xylose-induced promoter.
Xylose induced promoter XylA1 is weaker than arabinose and rhamnose promoters.
Characteristics and Results
Legend:
XylWT (XylA) [BBa_K1741007]
XylA1 [BBa_K1741008]
XylS [BBa_K1741009]
XylF [BBa_K1741010]
All our xylose promoters are induced by xylose and repressed by glucose. We also observed slight induction by arabinose.
We observed that XylA1 and XylF had a lower expression than XylWT. XylS is the strongest xylose-induced promoter. The sfGFP fluorescence [RFU] was measured using Tecan fluoremeter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 153
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 419
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