![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K1820003
Designed by: Thomas Overbeck Group: iGEM15_Utah_State (2015-09-16)
Revision as of 19:45, 18 September 2015 by Tom.overbeck (Talk | contribs)
P335-Φ31phage genetic switch-divergent promoter + native RBS
A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31 (BBa_K1820002), with the native ribosomal binding site (RBS)
Usage and Biology
This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes, and translation beginning at the RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.
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Categories
Parameters
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