Generator

Part:BBa_K1709003

Designed by: Astrid Deryckere   Group: iGEM15_KU_Leuven   (2015-09-16)
Revision as of 10:24, 16 September 2015 by Registry (Talk | contribs)

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CheZ-GFP

Upon recognition of a small molecule (e.g. nutrients or toxins), chemoreceptors transduce a signal through a set of Che proteins. This group of methylesterases and phosphatases regulates the rotation of the flagella. At the end of the chain of Che proteins is CheY. In phosphorylated state, CheY binds the flagella and causes the cells to tumble. The phosphatase CheZ dephosphorylates CheY, inducing dissociation of CheY from the flagella enabling the cells to swim. Cells lacking the CheZ protein are unable to swim and will tumble excessively and incessantly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1307


[edit]
Categories
//cds/reporter/gfp
//chassis/prokaryote/ecoli
//direction/forward
//function/motility
//rbs/prokaryote/constitutive/anderson
Parameters
chassisE. Coli
directionForward
excitation
functionChemotaxis regulator: dephosphorylation of CheY to enable swimming of E. Coli. Fused to GFP for visualisation
proteinCheZ-GFP
rbs
tagLVA