RNA

Part:BBa_K1645998:Experience

Designed by: Peivand Sadat Mousavi   Group: iGEM15_Waterloo   (2015-09-18)
Revision as of 12:53, 26 September 2015 by Peivandsm (Talk | contribs) (→‎Applications of BBa_K1645998)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

BBa K1645998-Experience.png===Applications of BBa_K1645998=== This part can be used along with Cas9 once it is placed in front of an appropriate promoter in order to suppress transcription of a gene that is under lacI promoter. Our experiment was conducted in BL21. The characterization data using this part from our experiments demonstrates that when using this sgRNA co-transformed with dCas9, the RFP intensity which uses the LacI promoter is being suppressed by 41% compared with our control RFP not containing cas9 system. Here is the Flow Cytometry results, where the grey is control and blue is this biobrick used with dCas9. Significant repression of RFP can be seen in this graph.

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UNIQ80da2046debe5964-partinfo-00000000-QINU UNIQ80da2046debe5964-partinfo-00000001-QINU