Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Coding

Part:BBa_K1645999

Designed by: Peivand Sadat Mousavi   Group: iGEM15_Waterloo   (2015-09-17)
Revision as of 01:02, 18 September 2015 by Peivandsm (Talk | contribs)

Dual Color Plasmid (RFP and GFP)

This part is the composite part of GFP full cassette taken from registry part BBa_I20260 and RFP full cassette taken from registry part BBa_J04450. The GFP is under the J23101 promoter, which is a constitutive promoter. In addition, the RFP is under the lacI (R0010) promoter which is constitutive in DH5-alpha due to the absence of lacI expression. If lacI repressor is presented in the cell the RFP expression is inhibited and IPTG is needed for the expression of RFP. For our purposes, this part was used to see the colour swaps using different SgRNA targeting promoter region of each of the colours.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1708
    Illegal AgeI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706


[edit]
Categories
Parameters
None