Coding

Part:BBa_K1680007

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 01:02, 26 September 2015 by KatharinaS (Talk | contribs) (→‎Sequence and Features)

Cre recombinase

This part contains the protein coding sequence for the DNA recombinase Cre and is codon optimized for yeast. Cre recombinases recognize two loxp sites (BBa_K1680005) and depending on their orientation cuts out or reverses the DNA sequence between them.


Sequence and Features

This part is submitted in pSB1C3 with RFC25 standard. The part only sequence does therefore not contain a start and stop codon.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 437


[edit]
Categories
//cds
//chassis/eukaryote/yeast
//function/recombination/cre
Parameters
None