Plasmid_Backbone
Part:BBa_K1680016:Design
Designed by: Katharina Sporbeck Group: iGEM15_Tuebingen (2015-09-17)
pRS316 yeast shuttle vector with Biobrick MCS
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2040
Illegal XbaI site found at 2025
Illegal SpeI site found at 2017
Illegal PstI site found at 2003 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2040
Illegal SpeI site found at 2017
Illegal PstI site found at 2003
Illegal NotI site found at 2008
Illegal NotI site found at 2032 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2040 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2040
Illegal XbaI site found at 2025
Illegal SpeI site found at 2017
Illegal PstI site found at 2003 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2040
Illegal XbaI site found at 2025
Illegal SpeI site found at 2017
Illegal PstI site found at 2003
Illegal NgoMIV site found at 1668 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1079
Illegal BsaI.rc site found at 3414
Illegal SapI site found at 2331
Illegal SapI site found at 4435
Illegal SapI.rc site found at 926
Design Notes
Has an RFC10 compatible MCS, with ADH Terminator. No further RFC10 restrictions sites in the backbone.
Source
yeast shuttle vector