Reporter

Part:BBa_K1680025

Designed by: Nicolai von Kuegelgen   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 14:52, 17 September 2015 by Registry (Talk | contribs)

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RFP-luciferase Cre reporter

This part encodes a reporter cassette for the Cre Recombinase (e.g parts XYZ) in Yeast. An active Cre protein will switch expression from RFP to NanoLuc luciferase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
    Illegal AgeI site found at 2048
    Illegal AgeI site found at 2160
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830


[edit]
Categories
//chassis/eukaryote/yeast
//function/recombination/cre
//function/reporter/fluorescence
//function/reporter/light
Parameters
None