Coding

Part:BBa_K1850013

Designed by: Lydia Goldberg   Group: iGEM15_Harvard_BioDesign   (2015-09-15)
Revision as of 00:45, 24 September 2015 by Sandias42 (Talk | contribs)

pAra - fim pAra fim contains the fim operon, including the structural proteins, transporters, and transmembrane domain of Type 1 Pili under titratable control of the pBad arabinose promoter BBa_I13453. When switched into an expression backbone and induced with a correctly expressed fimH variant (see BBa_K1850000, BBa_K1850002-11), pAra fim will produce Type 1 Pili with desired fimH adhesin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 998
    Illegal AgeI site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None