Composite
Part:BBa_K1618046
Designed by: Kieran Rustage Group: iGEM15_NRP-UEA-Norwich (2015-09-14)
THL.BHBD.CRO.BCD.EFb.EFa.BUK.PTB
.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 229
Illegal suffix found in sequence at 7178
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 7222
Illegal SpeI site found at 8122
Illegal PstI site found at 8136 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal NheI site found at 1167
Illegal SpeI site found at 7179
Illegal SpeI site found at 8122
Illegal PstI site found at 7193
Illegal PstI site found at 8136
Illegal NotI site found at 235
Illegal NotI site found at 3841
Illegal NotI site found at 7186
Illegal NotI site found at 7213
Illegal NotI site found at 8129 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal BglII site found at 1415
Illegal BglII site found at 6388
Illegal BglII site found at 6628
Illegal BglII site found at 6769
Illegal BglII site found at 7546 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 229
Illegal suffix found in sequence at 7179
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 7222
Illegal SpeI site found at 8122
Illegal PstI site found at 8136 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 229
Illegal EcoRI site found at 3159
Illegal EcoRI site found at 7207
Illegal XbaI site found at 244
Illegal XbaI site found at 7222
Illegal SpeI site found at 7179
Illegal SpeI site found at 8122
Illegal PstI site found at 7193
Illegal PstI site found at 8136
Illegal AgeI site found at 1338
Illegal AgeI site found at 3354 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7372
[edit]
Categories
Parameters
None |