Composite

Part:BBa_K1618017

Designed by: Kieran Rustage   Group: iGEM15_NRP-UEA-Norwich   (2015-08-28)
Revision as of 13:43, 22 September 2015 by Flaffycat (Talk | contribs)

Plac-BCD

K1618014 contains the coding sequence for the enzyme Butyryl-CoA dehydrogenase (BCD) of the butyrate biosynthetic pathways (Figure 1) from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. Thiolase catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA

NRP-UEA-Norwich-Flavs-Thing.png

Figure 1: The butyrate biosynthetic pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 278
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 278
    Illegal NotI site found at 960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 278
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 278
    Illegal AgeI site found at 473
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None