Not Released
Sample In stock
Experience: Issues
Not Used
Get This Part
Generator

Part:BBa_K1859023:Experience

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)
Revision as of 09:23, 22 September 2015 by Kozakai (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

===Applications of BBa_K1859023===

We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane H shows this sample.


left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay

There was no fluorescence. We found that this generator doesn’t make functional long chain protein.


User Reviews

UNIQa26ea2fa278b5a88-partinfo-00000001-QINU UNIQa26ea2fa278b5a88-partinfo-00000002-QINU