Generator

Part:BBa_K1859026

Designed by: Yusuke Banno   Group: iGEM15_Gifu   (2015-09-10)
Revision as of 08:29, 22 September 2015 by Nomuken (Talk | contribs)

mRNA circularize efficiently generator (outside complementarity)

We designed this generator [K1859026] to improve circular efficiency of mRNA including His-RFP sequence.

First, we improved Circular Parts of iGEM2014,the 5´side of the intron [BBa_K1332003] and the 3´side of the intron [BBa_K1332005] , and made [BBa_K1859015] and [BBa_K1859016] . Circular parts of iGEM2014 were cloned only sequence of ribozyme involved the splicing from td intron in T4 phage. However, [BBa_K1859015] and [BBa_K1859016] were cloned the sequence extra in addition to the ribozyme .

The generator is comprised of Circular parts improved to become complementary ( [BBa_K1859015] and [BBa_K1859016] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Tne RNA made by this generator [BBa_1859026] was designed to bind outside of circular mRNA complementary.

We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators [BBa_K1332011] , [BBa_K1859024] , [BBa_K1859025] ,[BBa_K1859026].

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