Composite

Part:BBa_K1615060

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-28)
Revision as of 14:58, 28 August 2015 by Registry (Talk | contribs)

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HerE fused to Linker+dCBD driven by LacI promoter

Heroin Esterase fused N terminally to dCBD + N terminal linker driven by the IPTG inducible PLlac 0-1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1674
    Illegal BamHI site found at 2430
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None