Regulatory
mLacI
Part:BBa_K1639011
Designed by: Nurgeldi Bazarov Group: iGEM15_ATOMS-Turkiye (2015-09-15)
mammalian LacI with miR-223 and miR-21 binding sites
In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-223 and miR-21 levels. miR-21 has high rate of production in most cancer types. In gastric epithelial cells level of this miRNAs are low but in gastric cancer cells their level is very high.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1172
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1201
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 562
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 787
Illegal BsaI.rc site found at 1165
[edit]
Categories
Parameters
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