Regulatory
mLacI

Part:BBa_K1639010

Designed by: Nurgeldi Bazarov   Group: iGEM15_ATOMS-Turkiye   (2015-09-15)
Revision as of 21:50, 21 September 2015 by Nurish (Talk | contribs)

mammalian LacI with miR-373 binding site

In our project second step of cancer module is based on production of LacI so that it will suppress expression of DsRed. Binding site on 3' end of LacI makes it regulatable and limits it's expression only in cells with low miR-373 rate. In gastric epithelial cells level of this mirna is low but in gastric cancer cells level is very high.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1170
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1193
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 787
    Illegal BsaI.rc site found at 1186


[edit]
Categories
Parameters
None