![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1616013
Designed by: Johanna Chesnel Group: iGEM15_IONIS_Paris (2015-09-18)
ccdB reversed - RBS and double T7 terminator
ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison. The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.
Usage and Biology
This part can be used with a reversed promoter to induce cell lysis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 226
Design Notes
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.
Source
This part is composed of ccdB reversed, RBS reversed and the double terminator T7 reversed.
[edit]
Categories
Parameters
//biosafety/kill_switch
None |