Coding
H/E-rev

Part:BBa_K1616005

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-17)
Revision as of 16:59, 17 September 2015 by Chesne j (Talk | contribs) (Created page with "__NOTOC__ <partinfo>BBa_K1616005 short</partinfo> H/E Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates ho...")

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Holin/endolysin reversed - RBS and double T7 terminator


H/E Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bläsi, 1995)




Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Check of illegal sites


Source

BBa_K1616005 is a composite part with BBa_K1616006 (endolysin reversed), BBa_K1616007 (reversed), BBa_K1616011 (RBS reversed) and BBa_B0025 (Double terminator T7 reversed).

References

Young, R., & Bläsi, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191–205). http://doi.org/10.1016/01686445(94)00079-4

[edit]
Categories
//biosafety
//biosafety/kill_switch
Parameters
None