Coding
ProGlgBRv3

Part:BBa_K1618027

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-09-03)
Revision as of 14:45, 9 September 2015 by Cym13puu (Talk | contribs)

GlgB-Rv3037c with IPTG-inducible promoter

Rv3037c is a genetic sequence which encodes for a putative acyltransferase enzyme which will hopefully acylate glycogen. This is preceded by GlgB which encodes for a glycogen branching enzyme which cleaves alpha-1,4 linkages in glycogen and re-anneals the cleaved strand onto the main glycogen strand to create an alpha-1,6 linkage to create a branch. Promoted by an IPTG-inducible promoter and encoded within the standard pSB1C3 vector. The bacteria are therefore chloramphenicol resistant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 3142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2952
    Illegal NgoMIV site found at 3125
    Illegal NgoMIV site found at 3449
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917


[edit]
Categories
Parameters
None