Coding

Part:BBa_K1850003

Designed by: Lydia Goldberg   Group: iGEM15_Harvard_BioDesign   (2015-09-15)
Revision as of 19:27, 20 September 2015 by Sandias42 (Talk | contribs)

pRha - fimH - SpyTag_225 - HisTag_258

Titratable Rhamnose promtoter with fimH gene and HisTag insertion at position 258 for nickel binding. SpyTag was added for use with binding partner Spycatcher but not experimentally validated. This part is for the production of modified type 1 pili.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Zakeri, B., Fierer, J., Celik, E., Chittock, E., Schwarz-Linek, U., Moy, V., & Howarth, M. (2012). Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proceedings of the National Academy of Sciences.

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