DNA
Part:BBa_K1614025
Designed by: Daniel Heid Group: iGEM15_Heidelberg (2015-09-18)
Ketamin-dependent self cleaving F8 - readout DNAzyme
Construct consisting of a ketamin-switched F8 DNAzyme linked to a stemmed HRP mimicking DNAzyme.
If there no ketamine binds to aptamer the F8 is inactive and HRP mimicking DNAzyme is stemmed, so that no hemin could bind and there is no peroxidase activity.
By binding of ketamin to the aptamer, F8 is activated and cleaves the stemming part, so the HRP mimicking DNAzyme is activated, peroxidase activity could be used for different readout techniques.
Stems and Aptamer generated by MAWS and JAWS software.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |